anti srf (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti srf
Anti Srf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srf+antibody/pm41861925-89-9-11
Average 86 stars, based on 1 article reviews
Anti Srf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srf+antibody/pm41861925-89-9-11
Average 86 stars, based on 1 article reviews
anti srf - by Bioz Stars,
2026-10
86/100 stars
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Chromatin Immunoprecipitation:Article Title: Nutrient regulation of the islet epigenome controls adaptive insulin secretion Article Snippet: Lsd1 ChIP of islets from fed or fasted mice was performed as described (69) following nuclear isolation of 1,000 dissociated islets using 30 μL of Dynabeads Protein A beads (Life Technologies) conjugated to 4 μg anti-Lsd1 antibody (ab17721, Abcam) in a total volume of 120 μL. .. The same protocol was followed for Srf ChIP-Seq in Min6 cells with the following modifications: 1.25 × 107 cells were used for immunoprecipitation using 4 μl of Article Title: Profiles of novel high-molecular-weight synthetic antioxidants in urine and associated child exposure in China. Article Snippet: 1 Introduction The ability to regulate nutrient metabolism in response to feeding and fasting is necessary for metabolic homeostasis.. Nutrient utilization is acutely regulated by hormones and metabolites that change in response to feeding state (1).. If an energy state persists, adaptive control mechanisms increasingly influence nutrient metabolism. Article Title: Nutrient regulation of the islet epigenome controls adaptive insulin secretion Article Snippet: Lsd1 ChIP of islets from fed or fasted mice was performed as described ( ) following nuclear isolation of 1,000 dissociated islets using 30 μL of Dynabeads Protein A beads (Life Technologies) conjugated to 4 μg anti-Lsd1 antibody (ab17721, Abcam) in a total volume of 120 μL. .. The same protocol was followed for Srf ChIP-Seq in Min6 cells with the following modifications: 1.25 × 10 7 cells were used for immunoprecipitation using 4 μl of Immunoprecipitation:Article Title: Nutrient regulation of the islet epigenome controls adaptive insulin secretion Article Snippet: Lsd1 ChIP of islets from fed or fasted mice was performed as described (69) following nuclear isolation of 1,000 dissociated islets using 30 μL of Dynabeads Protein A beads (Life Technologies) conjugated to 4 μg anti-Lsd1 antibody (ab17721, Abcam) in a total volume of 120 μL. .. The same protocol was followed for Srf ChIP-Seq in Min6 cells with the following modifications: 1.25 × 107 cells were used for immunoprecipitation using 4 μl of Article Title: Profiles of novel high-molecular-weight synthetic antioxidants in urine and associated child exposure in China. Article Snippet: 1 Introduction The ability to regulate nutrient metabolism in response to feeding and fasting is necessary for metabolic homeostasis.. Nutrient utilization is acutely regulated by hormones and metabolites that change in response to feeding state (1).. If an energy state persists, adaptive control mechanisms increasingly influence nutrient metabolism. Article Title: Nutrient regulation of the islet epigenome controls adaptive insulin secretion Article Snippet: Lsd1 ChIP of islets from fed or fasted mice was performed as described ( ) following nuclear isolation of 1,000 dissociated islets using 30 μL of Dynabeads Protein A beads (Life Technologies) conjugated to 4 μg anti-Lsd1 antibody (ab17721, Abcam) in a total volume of 120 μL. .. The same protocol was followed for Srf ChIP-Seq in Min6 cells with the following modifications: 1.25 × 10 7 cells were used for immunoprecipitation using 4 μl of Article Title: Inhibition of TRPM7 blocks MRTF/SRF-dependent transcriptional and tumorigenic activity. Article Snippet: Myocardin-related transcription factors A and B (MRTFs) are coactivators of Serum Response Factor (SRF) that mediates the expression of genes involved in cell proliferation, migration and differentiation.. There is mounting evidence that MRTFs and SRF represent promising targets for hepatocellular carcinoma (HCC) growth.. Since MRTF-A nuclear localization is a prerequisite for its transcriptional activity and oncogenic properties, we searched for pharmacologically active compounds able to redistribute MRTF-A to the cytoplasm. Cell-Signaling:Article Title: Human umbilical cord/placenta-derived mesenchymal stem cell secretome attenuates intestinal fibrosis Article Snippet: The following antibodies were used: procollagen1A1 (Procol1A1) antibody (SP1D8, Developmental Studies Hybridoma Bank, Iowa City, IA) and bronectin (FN) antibody (ab2413, Abcam, Cambridge, MA). .. Other include α-smooth muscle actin (α-SMA) antibody (A2547, Sigma), phospho-Smad2 (Ser465/467) antibody (#3108, Cell Signaling), RhoA antibody (#sc-418, Santa Cruz Biotechnology, Dallas, TX), GAPDH antibody (#2118, Cell Signaling), Mkl1(MRTF-A) antibody (21166-1-AP, ProteinTech), Article Title: Human umbilical cord/placenta mesenchymal stem cell conditioned medium attenuates intestinal fibrosis in vivo and in vitro Article Snippet: The following antibodies were used: procollagen1A1 (Procol1A1) antibody (SP1D8, Developmental Studies Hybridoma Bank, Iowa City, IA) and fibronectin (FN) antibody (ab2413, Abcam, Cambridge, MA). .. Others include α-smooth muscle actin (α-SMA) antibody (A2547, Sigma), phospho-Smad2 (Ser465/467) antibody (#3108, Cell Signaling), RhoA antibody (#sc-418, Santa Cruz Biotechnology, Dallas, TX), GAPDH antibody (#2118, Cell Signaling), Mkl1(MRTF-A) antibody (21166-1-AP, ProteinTech), |
![Contractile Gene Expression Is Downregulated in Chol-Loaded hVSMCs (A, B) Human vascular smooth muscle cells (hVSMCs) were treated with cholesterol (Chol) (5 μg/mL) or 0.2% bovine serum albumin (control [CT]) for 24 hours and 48 hours and gene expression <t>of</t> <t>Acta2</t> , Tagln , Cnn1, Myocd, and <t>Srf</t> were determined by quantitative polymerase chain reaction. (C) hVSMCs were treated with Chol (5 μg/mL) or 0.2% bovine serum albumin (CT) for 24 hours and protein expression of α–smooth muscle actin (α-SMA) and CNN1 were determined by Western blotting (representative blots shown). Densitometry showing the (D) α-SMA and (E) CNN1 band intensities normalized to GAPDH. For data analysis, unpaired Student’s t -testing was performed for comparing the means of 2 groups. For 2 or more independent groups, 1-way analysis of variance followed by Dunnett post hoc test was performed. A P value of ≤0.05 was considered significant. Data are presented as the mean ± SEM of 3 independent experiments, and P values are as indicated (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5216/pmc12925216/pmc12925216__gr1.jpg)
